Refocusing on good microbiological practices and procedure. Biosafety and biosecurity in the veterinary microb iology laboratory and animal facilities 18 oie terrestrial manual 2012 v the statutory confinement of diseased, infected and incontact animals is necessary. The laboratory safety group within the environmental health and safety office ehs has developed the biological safety manual to provide guidance on the safe handling of biological materials, safety practices to minimize the risk of exposure to potentially infectious materials, and procedures for. Highrisk tb laboratories tbcontainment laboratories. Laboratory biosafety is the process of applying a combination of administrative controls, containment. The ibc advocates the use of biosafety precautions that effectively reduce or. The biosafety manual must be available and accessible.
The biosafety level bsl is a description of the degree of physical containment being employed to confine biohazardous material within a laboratory or facility and to reduce the potential for exposure of laboratory workers, persons outside of the laboratory, and the environment. This manual shall contain the pis ibc application and amendments as well as all ibc approval letters. This type of facility may or may not meet all of the requirements of a biosafety level 3 laboratory as described in whos laboratory biosafety manual. The purpose of this manual is to define the biological safety policies and procedures pertaining to research operations at boston university and boston medical center. Footnote 5 it is a guidance document for canadian facilities where human and terrestrial animal pathogens are handled, that updates the guidelines originally published as part ii of the. Wcln resources wisconsin state laboratory of hygiene.
Good microbiological technique and appropriate use of biosafety equipment by well trained staff remain the fundamental elements of laboratory biosafety. The standard bsl2 precautions are outlined in the universitys adopted biosafety manual cdcnih biosafety in microbiological and biomedical laboratories bmbl. A manual of recommended practice for design acgih 20, ansiaihaasse z9. The purpose of the uc irvine biosafety manual is to specify the practices, procedures. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Working practices including spills and aerosol generating activities are addressed. Canadian biosafety handbook, second edition canada. This manual describes the minimum biosafety measures that should be implemented at the different levels of. Guidelines for laboratory facility commissioning 8. Who laboratory biosafety manual updates absa conference. A laboratoryacquired infection is known or suspected, or b. Its primary focus should be to provide pertinent information to help execute the operations of the lab in a safe and professional manner. Tuberculosis laboratory biosafety manual v executive summary following a technical consultation held between the world health organization who and the united states centers for disease control and prevention cdc in atlanta, ga, in september 2008 on strategies, approaches and partnerships that could be implemented to improve laboratory biosafety.
The canadian biosafety handbook cbh, 2 nd edition, 2016 is intended as a companion document to the canadian biosafety standard cbs, 2 nd edition, 2015. It is therefore an imperative that laboratory operations adhere to best practices and. Pdf tuberculosis laboratory biosafety manual lasinrang. A pathogen that meets one of the following criteria.
Laboratory biosafety manual world health organization. Sop 900 approval process for inactivation methods for infectious material. Who mycobacteriology biosafety manual 2012 handbook on tb laboratory diagnostic methods for european union2018 efficacy and safety of delamanid in combination with an optimised background regimen for treatment of multidrugresistant tuberculosis. Case western reserve university laboratory biosafety manual. Manual, the us biosafety in microbiological and biomedical laboratories, and the international consensus document cen workshop agreement cwa 15793. Biological safety training manual ubc risk management. Biosafety practices and procedures biosafety program. Tuberculosis laboratory biosafety manual vii abbreviations ach air. Thus no special equipments are in place to prevent biohazards.
Classification of laboratory ventilation design levels developed by ashrae technical committee 9. Procedures that involve aerosols or high splash potential that may increase the risk of personnel exposure shall be conducted in primary containment equipment class i or class ii biosafety cabinet bsc. University of illinois at chicago uicehso 2012 biological safety program manual page 30 of 96 materials such as autoclave tape, must be used with each load. Guidance documents manuals ebsa european biosafety. Who laboratory biosafety manual, 3rd edition risk assessment. Tuberculosis laboratory biosafety manual laboratory biorisk management. Center for vaccine biology and immunology cell sorting. Laurier university for giving permission to use their biosafety manual as a template for this manual. The manual is based upon the health canada laboratory guidelines.
Formaldehyde in a concentration of 5% active ingredient is an. Laboratory guidelines research institute for tropical medicine. The who has issued a request for feedback on the new fourth edition of the who laboratory biosafety manual lbm. The laboratory biosafety manual should be a living, working document that is an important resource for personnel engaged in the activities using biological and or rdna materials. Typically, institutions that experiment with or create potentially harmful biological material will have a committee or board of supervisors that is in. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the comissioning and. Biosafety manual oklahoma state universitystillwater.
The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with bsl2. Managing the risks in laboratories and healthcare premises, hse, u. Risk assessment and the classification of tb laboratories. Biosafety biosafety manual thompson rivers university. Cdc biosafety in microbiological and biomedical laboratories bmbl 5th edition, cdc, u. The challenge for managers of tb programmes and staff at laboratories, particularly in resourcelimited. Laboratory quality standards and their implementation. Laboratory biosafety manual were published in 1993 and 2004 respectively 2,3.
University of california, irvine environmental health and safety. Canadian biosafety standard cbs second edition, canada biological agents. Biosafety manual office of environmental health and safety. The development of this fourth edition of the laboratory biosafety manual has. A spill of any quantity involving an agent infectious to humans, plants, or animals occurs in a public area.
Biosafety in microbiological and biomedical laboratories, 5th edition nih design requirements manual drm, 2008. Biosafety level1 represents a basic level of containment that relies on standard microbiological practices with no special primary or secondary barriers recommended, other than a sink for hand washing. This cannot be worn outside of the laboratory and must be discarded or decontaminated after each use. Guide for the care and use of laboratory animals, 8th edition. This biosafety manual is applicable to all laboratory, research, teaching, service, and support activities that may involveexposure to biohazard agents or materials ous or otherwisecomes under the purview of the institutional biosafety committee ibc at the university of montana. Guidelines for laboratory facility certification part ii. Case western reserve university laboratory biosafety manual rev 42012. The levels of containment range from the lowest biosafety level 1 bsl1 to the highest at level 4 bsl4. A laboratory specific biosafety manual must be drafted which details how the laboratory will operate in compliance with all safety requirements. Biosafety level refers to the stringency of biocontainment precautions deemed necessary by the centers for disease control and prevention cdc for laboratory work with infectious materials. The goals of the biological safety program, referenced in this manual as the biosafety program, are to protect laboratory workers, the public, and the environment from potentially hazardous biological agents.
These may react with organic materials such as paper. Tuberculosis laboratory biosafety manual world health organization. For this new edition, the manual has been extensively revised and expanded. Ebsa asks all members to send your comments to the document before tuesday may 28, 2019 by using the form below the lbm consists of a core. Biosafety and laboratory biosecurity, 2012 world health organization who biosafety in microbiological and biomedical. Laboratory personnel must wear solidfront protective clothing i. Biosafety and laboratory biosecurity, 2012 world health organization who biosafety in microbiological and biomedical laboratories, 5th edition, revised 2009 pdf, 260 kb. Laboratory biosafety manual third edition for more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Guidelines for safe work practices in human and animal. A core principle of biosafety is the containment of microorganisms. Foreword this reference manual outlines the safe use, storage, handling, waste disposal and emergency management of biohazardous materials for the university of northern british columbia unbc. Link to view o n l i n e american biological safety association absa link to view american society for clinical pathology ascp link to view.
The members of this panel recom mended that biosafety guidelines be developed to address the unique operational needs of the diagnostic laboratory community and that they be science based and made available broadly. Who laboratory biosafety manual, 3rd pdf icon external icon who laboratory biosafety guidance related to the novel coronavirus 2019ncov world health organization pdf icon external icon cdc 2007 guideline for isolation precautions. A biosafety level bsl, or pathogenprotection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. The maximum containment laboratory biosafety level 4 6. Openfronted class i and class ii biological safety cabinets. The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with bsl2 agents.
We promote biological safety at the nih through a broad range of support services, consultation and assistance. The who laboratory biosafety manual 4th edition youtube. Receive training in standard microbiological techniques. Mdh laboratories administration laboratory safety manual. O four biological risk groups list of illustrations 1.
The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to develop and establish national codes of practice for securing microbiological assets, yet ensuring their availability for clinical, research and epidemiological purposes. A manual of written standard operating procedures sops for the laboratory, in combination with this manual and the cdcnih publications biosafety in microbiological and biomedical laboratories, 5th edition and guidelines for research involving recombinant or synthetic nucleic acid molecules, provide general requirements for working with biological agents. The fourtier classification system of biosafety levels 14 described in whos laboratory biosafety manual 2 provides broad guidance on basic concepts of biosafety for the development of national and international codes of practice. Tuberculosis laboratory biosafety manual ncbi bookshelf. Who laboratory biosafety manual having served for global biosafety community hereinafter, who manual for more than 20 30 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. It is intended to inform rather than replace countrylevel requirements and standards for biosafety. Health laboratory services provide vital support for disease prevention, diagnosis, treatment management, screening and surveillance. Performing a risk assessment laboratory biosafety manual, 3rd edition, 2004 link to world health organization website guidelines for safe work practices in human and animal medical diagnostic laboratories link to cdc website. A laboratoryspecific biosafety manual must be prepared and adopted as policy.
Manual this manual does not address chemical, physical andor radiation hazards that are commonly encountered in a laboratory setting and is to be regarded as an addendum to the unbc chemical laboratory safety and methodology manual. Laboratory biosafety and biosecurity risk assessment. Laboratory safety manual university of california riverside main campus overview introduction the risks associated with laboratory research hazards are greatly reduced or eliminated when proper precautions and practices are observed. Laboratory biorisk management for laboratories handling. Specifically, this document addresses considerations. Jul 18, 2019 the who laboratory biosafety manual 4th edition. University of illinois at chicago uicehso 2012 biological safety program manual page 81 of 96 called paraformaldehyde.
In the united states, the centers for disease control and prevention cdc have specified these levels. A laboratory specific biosafety manual must be prepared and adopted as policy. Laboratory biosafety is the process of applying a combination of administrative controls, containment principles, practices and procedures, safety equipment, emergency preparedness, and facilities to enable laboratory staff to work safely with potentially infectious microorganisms. Preventing transmission of infectious agents in healthcare settings pdf icon. Manual on collection, storage, and transport of specimens for tb testing producing reliable test results does not rely solely on the right performance of the stepbystep procedures of the test itself. Biosafety manual the princeton university biosafety manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards. The term tbcontainment laboratory refers to a facility that has the minimum design features necessary to safely manipulate tb cultures.
Laboratory safety the handbook global edition stop tb. Laboratory safety nanomaterials, schools, hazard analysis. Biosafety in microbiological and biomedical laboratories 5th ed. Ventilated workstation manual for afb smear microscopy. It all starts in the acceptance of correct, adequate and quality specimens brought to the laboratory. Bsl1 practices, safety equipment, and facility design and construction are. Sample laboratory biosafety manual for microbiological activities. Who guidance on regulations for the transport of infectious substances, 20 2014 whohsegcr 2012. The who tuberculosis laboratory biosafety manual 2012 incorporated a risk assessment approach that considered the procedures being performed. Stop tb partnership global laboratory initiative gli.
882 490 739 815 696 919 969 838 727 1225 1168 834 65 882 208 132 337 1247 931 1157 302 794 181 95 1300 868 15 1040 1264 515 1409 1485 1132 1392 800 1113 233 443 126